Three-dimensional scaffolds as a model system for neural and endothelial "in vitro" culture

Biomaterials based on the hydrophobic homopolymer poly(ethyl acrylate), PEA, and its copolymers with hydroxyethyl acrylate, p(EA-co-HEA) and methacrylic acid, p(EA-co-MAAc) were prepared as polymeric scaffolds with interconnected pores of 90 microns and tested in vitro as culture substrates and compared for their impact on the differentiation of neural stem cells (NSC) obtained from the subventricular zone (SVZ) of postnatal rats and human endothelial cells (HUVEC). Immunocytochemical staining assay for specific markers show that p(EA-co-MAAc) scaffolds were suitable substrates to promote cell attachment and differentiation of adult NSC and HUVEC cells.Spanish Ministry of Science through Project No. MAT2007-66759-C03-01 (including the FEDER financial support) is acknowledged. Cope´rnicus-Santander (PRCEU-UCH/COP01/08) Universidad CEU- Cardenal Herrera. Programa de ayudas a la Investigacio´n Universidad CEU- Cardenal Herrera (PRCEU-UCH16/ 09). Programa de ayudas a la Investigacion cie´ntifica Ramo´n Areces (2009)